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So far I have been going through all possibilities when I can choose Investigate when talking to characters. Can this have a negative effect at any point or should I just go for it?

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pretty sure it doesn't ever, but this is sort of hard to answer definitively – Ben Brocka Mar 13 '12 at 0:18
It never did in ME or ME2. – Steve V. Mar 13 '12 at 0:26
@BenBrocka It is only hard to answer definitively if the answer is it can not have negative effects, by the looks of it that is the answer – Blem Mar 13 '12 at 0:45
up vote 1 down vote accepted

While I have no hard evidence to support this (not sure any exists without looking at the game code) in all my playthroughs there was never a negative effect for investigating/exploring all dialog. Same goes for talking to people just to hear the same thing over and over again.

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Investigate simply gives you additional background about the story and its characters. It is neither required for good outcomes, nor can it cause bad outcomes.

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