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linked to video that answers the question, since my original link is gone
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Adeese
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According to this thread at Xbox360Achievements.orgAccording to this thread at Xbox360Achievements.org, kills in the opening sequence do count towards the achievement.

Since my original link died, the IGN Strategize on Deus Ex: Human Revolution also states that kills induring the opening (pre-augmentation) sequence do count towardsagainst the Pacifist achievement.

According to this thread at Xbox360Achievements.org, kills in the opening sequence do count towards the achievement.

According to this thread at Xbox360Achievements.org, kills in the opening sequence do count towards the achievement.

Since my original link died, the IGN Strategize on Deus Ex: Human Revolution also states that kills during the opening (pre-augmentation) sequence do count against the Pacifist achievement.

edited body
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bwarner
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According to this thread at Xbox360Achievements.org, kills in the opening sequence todo count towards the achievement.

According to this thread at Xbox360Achievements.org, kills in the opening sequence to count towards the achievement.

According to this thread at Xbox360Achievements.org, kills in the opening sequence do count towards the achievement.

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Adeese
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According to this thread at Xbox360Achievements.org, kills in the opening sequence to count towards the achievement.