The Freeflow Perfection trophy/achievement in Batman: Arkham Asylum requires you to use all of Batman's combat moves in one freeflow combination. What are all these moves? What controller buttons are used to make them?
The moves you have to perform in a single combo are (PS3 / 360 / PC):
Strike (Square / X / Mouse Left)
Counter (Triangle / Y / Mouse Right)
Cape Stun (Circle / B / Mouse Middle)
Evade (X x2 / A x2 / Space x2)
Throw (Square + X / A + X / Shift + Mouse Left)
Takedown (Triangle + Circle / B + Y / Shift + Mouse Right)
Ground Takedown (R2 + Triangle / RT + Y / Ctrl Left + Mouse Right)
Batarang (L1 / LT / Q)
Batclaw (R2 x2 / RT x2 / C)
As far as which order and where to do it, I would check sites like True Achievements (Xbox 360), or PS3 Trophies Forums for some tips. Check YouTube as well, there's loads of videos for it and a bunch of different possible solutions.