When you pick up Liposuction, it notes that it lasts for 45 seconds. Does that 45-second timer pause when you enter an area like a shop/treasure room which causes the main timer to pause?


No*, unfortunately. The timer will continue while inside of a shop.

*I tested this by picking up Liposuction then waiting in a shop. The Liposuction ended up expiring while I was in the shop.

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